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Xylene Cyanol FF is used as histological stain and as tracker dye in DNA sequencing and electrophoresis.General description. Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.
Applications of gel electrophoresis In the separation of DNA fragments for DNA fingerprinting to investigate crime scenes. To analyze results of polymerase chain reaction. To analyze genes associated with a particular illness. In DNA profiling for taxonomy studies to distinguish different species.
Researchers use electrophoresis to test variations of vaccines with different levels and types of antibodies to conduct studies to find the best possible version of a single vaccine. Analysis of proteins and antibodies: Another key application for electrophoresis is protein and antibodies analysis
Methyl Orange-Xylene Cyanol, Indicator Solution, also known as Acid Blue 147, is used as a tracking dye and monitors the process of polyacrylamide gel electrophoresis and agarose gel electrophoresis and is used in titrations as a pH indicator.