Product code RXSOL-60-6605-242Category Lab Chemical

Price and availability on request. Send us the quantity and delivery location in Oman.

Product description

Ponceau S is a anionic dye commonly used for the detection of electro- blotted proteins after an electrophoretic separation prior to Western blotting [12–14] or MALDI-MS analysis [15].Ponceau S Staining Solution is supplied as ready to use. This product is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. This staining technique is often utilized to confirm protein electrotransfer in Western blotting assays prior to antibody-based detection.

Applications

Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*.

Dosage

Place the blot transfer membrane in a plastic box and rinse it with water three times, 5 minutes each. Stain the membrane with Ponceau S stain for 30 seconds to 1 minute. Destain the membrane with several changes of water for 30 seconds to 1 minute each, then air dry

Also known as

PONCEAU S FOR ELECTROPHORESIS

Notes

Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.) Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v).

Where we supply

Oman. Stocked at our warehouse in Barka, South Al Batinah, and supplied to Muscat, Ruwi, Mutrah, Seeb, Rusayl, Barka, Sohar, Shinas, Saham, Buraimi, Nizwa, Ibri, Sur, Salalah, Duqm, Khasab and every other governorate.

Ports and free zones. Port Sultan Qaboos (Mutrah), Mina Al Fahal, Sohar Port and Freezone, Port of Salalah and Salalah Free Zone, Port of Duqm and the Duqm Special Economic Zone, Port of Sur, Khasab Port and Al Mazunah Free Zone.

Industrial areas. Rusayl, Sohar, Barka (Sanaya), Raysut (Salalah), Nizwa, Sur, Buraimi and Duqm industrial estates, Knowledge Oasis Muscat, and oil and gas sites across Oman.

Beyond Oman. We have branches in the UAE (including Fujairah) and in India (Kandla, Kolkata, Visakhapatnam, Chennai and Mumbai), and we also supply to South Africa.