Your One Stop for All Chemicals
Industrial • Marine • Cleaning
+968 9948 9269 / +968 9396 0629
WhatsApp / Call Us Anytime
omanchem.com@gmail.com
Drop Us a Line
Muscat, Sultanate of Oman
Locate Us
GET A QUOTE

Search form

×
Your shopping cart is empty.

THIOGLYCOLATE INDICATOR

Educational Use Only - Order On Request
THIOGLYCOLATE INDICATOR
Get MSDS Get Technical Bulletin Connect on WhatsApp Request Info
Product Code: RXSOL-62-6047-111

Categories

Test Kit For Water-62

Product Description

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron. It works by reducing insoluble ferric iron (Fe³⁺) to ferrous iron (Fe²⁺) and binding with it to form a highly visible purple complex.

Thioglycolic acid is used for the reduction of iron(III) to iron(II), and for pH adjustment. Thus only one reagent is required for analysis.

Product Short Description

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron.

Product Application

How the Thioglycolate Method Works

 

  • Reduction: Thioglycolic acid reduces oxidized ferric iron (Fe³⁺) into soluble ferrous iron (Fe²⁺).

    •  

  • Complexation: The ferrous iron reacts with the thioglycolate to form a purple or pink-colored ferrous thioglycolate complex.

    •  

  • Measurement: The intensity of the resulting color is directly proportional to the total iron concentration in the water sample

    • Typical Testing Procedure
    1. Color Comparison: After a brief waiting period (usually 3 to 5 minutes), the color of the sample is compared against a standard color chart or measured photometrically to determine the iron level in mg/L (or ppm).
    Advantages and Limitations
    • Interferences: The formulation used in many test kits is designed to minimize interferences from other heavy metals that might be present in the water sample

    Product Dose

    Typical Testing Procedure

     

  • Sample Preparation: A measured water sample is placed into a test tube or flask.

    •  

  • Reagent Addition: Citric acid is often added first to prevent the precipitation of iron, followed by the thioglycolate indicator.

    •  

  • pH Adjustment: Ammonia is added to make the solution alkaline. This step is crucial for the colored complex to form.

    Color Comparison: After a brief waiting period (usually 3 to 5 minutes), the color of the sample is compared against a standard color chart or measured photometrically to determine the iron level in mg/L (or ppm)

    •  

    Product Note

    A thioglycollate indicator (typically resazurin) is a redox dye used to visually map oxygen levels in bacterial culture tubes. 
    In its oxidized state, it turns pink when exposed to oxygen; in its reduced, oxygen-free state (typically at the bottom), it remains colorless.
     
    Resazurin Indicator Characteristics
    Pink Zone: Indicates the presence of oxygen (aerobic zone) at the top of the tube.
    Colorless Zone: Indicates the absence of oxygen (anaerobic zone) deeper in the tube.
    Reversibility: Resazurin is highly sensitive; if the broth is shaken, it re-oxidizes and the top portion will turn pink until the thioglycolic acid reduces the oxygen again

    Remarks

    2,4,6- Tris-(2’ - Pyridyl )-s-Triazine ( TPTZ ) or  also can be use like 2,4,6-Tripyridyl-s-triazine. Solution of (TPTZ), .001 M were prepared by dissolving 0.312 gram of compound in a view drops of hydrochloric acid and diluting to 1 ltr with water.

     
    2,4,6-Tripyridyl-s-triazine solution of (TPTZ) is useful for the calorimetric determination of iron. The Intense violet colour formed by TPTZ with iron(II) is due to the ion {Fe(TPTZ)2}+2, with only two of the three pyridyl group of each TPTZ ligand attached to the Iron atom.
     
    This reagent is  also used for determination of iron in silicates, burnt refractories, and limestone etc

    Other Equivalent Brand

    Thioglycolic Acid

    Free Delivery / Supply Locations

    Oman, Bahrain, Abu Dhabi, Ajman, Al Ain, Dubai, Ras Al-Khaimah, Fujairah, Sharjah, Umm Al Quwain, Ruwais, Khalifa Port, Jebel Ali, DP WORLD, and all UAE ports...